Figure 1.10: Titration
A known mass of material is homogenised in a saline solution to produce a 1 per cent suspension. After mixing, an aliquot is removed and diluted tenfold, to produce a 0.1 per cent solution. This procedure is repeated until the required dilution is reached. Aliquots of the required dilutions are then inoculated into experimental animals. The dilution (titre) which produces 50 per cent affected animals is the ID50 dilution, referred to as 1 unit of infectivity. This can also be expressed in terms of the equivalent weight of the starting material.
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